Rapid Immunological Tests for the Detection of Phytophthora Cinnamomi in Avocado Roots
نویسندگان
چکیده
We will first prepare reference antisera to total proteins of P. cinnamomi and P. citricola. The protein profile of each fungus will be analyzed by SDS-Polyacrylamide Gel Electrophoresis(PAGE). By this procedure, fungal proteins are driven by an electric current and are separated into bands in a gel medium. The distance of the movement of each protein is dictated by its size. The protein bands will again be transferred to a membrane by electric current and allowed to react with each reference antiserum. A protein band which does not react with the heterologous antiserum is identified as unique to itself.
منابع مشابه
South African Avocado Growers’ Association Yearbook 1990. 13:61-62 A PRELIMINARY REPORT The influence of N and Casources on pathogenicity of Phytophthora cinnamomi and of Ca-sources on resistance of avocado roots to infection by the fungus
Saprophytic growth of Phytophthora cinnamomi indicated that the pathogenicity was not enhanced by addition of Nor Ca-sources to the basal growth medium. Mycelium obtained from the N and Ca-enriched sources and used to inoculate blue lupin seedlings indicated that the pathogenicity of the fungus was reduced by the addition of either N or Ca to the fungus cultures. The detached root technique was...
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